Protein Absorption Myth: 20-30g Or More?

August 24, 2021

So, you’ve got your parents, significant other, and gym-bros telling you you are eating too much protein. They start to pull up websites like Men’s Health (yuck) to “prove” you can only absorb 20-30 g of protein per meal.

The myth of only being able to absorb 20-30 g of protein in one meal is just that, a myth. Research shows ingesting 40 g of protein after training can increase muscle protein synthesis greater than 20 g and those who intermittent fast maintain muscle mass when eating 54 g of protein per meal.

If we can absorb more than 20-30 g of protein per meal, where did this myth originate and how do we know how much we should eat to maximize adaptations to lifting weights?

The Effect Of Protein Absorption From One Meal

This protein myth came about from a single study where healthy subjects drank either 0g, 5g, 10g, 20 g, or 40 g of protein after resistance training [1].

They found that protein synthesis capped out at 20 g of protein so no further increase was seen when taking 40 g of protein.

Cue every mainstream fitness magazine telling you to only eat 20 g of protein at once.

However, we can't just take these results at face value. Many factors contribute to protein utilization within the body. These include [2]:

  • Composition of the protein (i.e. amino acids)
  • Composition of the meal (i.e. protein, carbs, and fats)
  • The amount of protein ingested (i.e. 20 or 40 g)
  • The exercise routine (i.e. mode, volume, intensity, etc)
  • Age
  • Training status
  • Amount of lean mass
Protein Absorption Myth

All of these will influence the rate of digestion of protein. It’s important to understand that protein that is digested very quickly (e.g. whey protein) can lower the total protein used as some amino acids are oxidized (i.e. not used to spike protein synthesis) when taken in large quantities.

As the absorption rate of fast-acting protein is capped at around 10 g per hour, taking in a lot more protein at once would lower the balance between absorbable protein and protein that is oxidized [2].

However, if the rate of absorption is slowed down by changing the composition of the meal, then we may potentially slow down this oxidation of amino acids leaving us with more protein to use [2].

Secondly, the resistance training routine in this study was 4 sets of leg press, leg extension, and leg curl for 8-10 reps to failure.

As Lift Big Eat Big followers, you will know that’s not a huge stimulus. If it was 4 sets of squats, it may be a different story.

Lastly, these six subjects weighed 86 kg (190 lbs) and had a height of 182 cm (5’11”). These were not very muscular individuals and resistance training experience doesn’t tell us much about their background. Unfortunately, strength measures were not recorded in this study.

A second study decided to follow up on these findings which took subjects of similar stature who performed 4 x 10 reps of the leg extension at 80% 1RM [3].

Following this, for 12 hours subjects were given 80 g of protein. One group drank this 10 g at a time every 1.5 hours. Another group drank it 20 g at a time every 3 hours while the final group drank 40 g at a time every 6 hours.

They found muscle protein synthesis was greatest in the 20 g group with no additional benefits in the 40 g group.

Again, there are severe limitations to this study similar to that of the last study including the resistance training routine (even less work than the previous study) and the subjects used.

Most glaringly are these subjects consumed ONLY 80 g of protein for 12 hours after training. No carbs. No fats.

Considering the dietary protein requirement for bodybuilders to maintain or build muscle mass is 1.7 - 2.2g/kg of body weight (0.8 - 1g/lb) [4], these subjects ate approximately 1g/kg of body weight. Literally half of the daily requirement.

So what happens when you take similar subjects used in these two studies as well as heavily muscled individuals, put them through a full-body routine with each exercise ending in a set to failure, then give them either 20 g or 40 g of whey protein [5]?

Funnily enough, both groups see greater muscle protein synthesis ingesting 40 g of protein compared to 20 g. 

It seems that the resistance training protocol has a seemingly large impact on protein absorption at least at the acute level.

Laura Semotiuk, Nutrition coach and author for FeastGood says:

The most important factor when it comes to protein consumption, is to get sufficient protein daily. To maximize protein absorption post-workout, opt for a meal that contains protein, carbs and fat. If you have a protein shake post-workout, stick to one scoop and consume a meal 1-1.5 hours later.

While understanding the short-term responses to protein ingestion, acute anabolic responses generally don't influence long-term muscular gains. Instead, it's the long game when it comes to building muscle.

Long Term Protein Measures

In the elderly, providing approximately 80% of their daily protein intake in one meal for 14 days retained greater fat-free mass compared to spreading it out over four meals indicating muscle mass is not negatively affected when consuming the majority of protein in one meal [6].

However, resistance training was not used in this study.

Yes, I can hear you now. “James, how do the elderly eating a large protein meal relate to a lifter like me?”

While it may not directly relate to you, it highlights the premise that only being able to absorb 20 g of protein in one sitting is likely false. If this was the case, we would see large losses in fat-free mass in these elderly subjects.

The trend of intermittent fasting also supports this study. One intermittent fasting study had subjects perform intermittent fasting every second day across 14 days from 10 pm to 6 pm the following day [7].

Macronutrients were split evenly between the three and caloric intake was matched in both intermittent fasting and standard meal conditions.

There was no difference between conditions for retaining muscle mass even though the intermittent fasting condition had subjects consume approximately 101 g of protein within 4 hours.

When we look at resistance-trained males who for 8 weeks ate in an 8-hour window with a 16 hour fast every day, they reduced fat mass while maintaining fat-free mass while the non-restricted group maintained both fat and fat-free mass [8].

These subjects were eating 1.9g/kg of body weight over 3 meals which equates to 0.63g/kg of body weight per meal. Well above the 20 g per meal recommendation which would equate to 0.23g/kg of body weight over 6 meals.

Protein intakes can go even higher than this and have consistently shown benefits when looking to retain muscle mass. Recommendations of 2.3-3.1g/kg (1.05-1.4g/lb) of body weight are the norm when looking to preserve muscle while dieting with individuals on the leaner side sitting higher up in the range compared to those with greater fat mass [9].

In fact, eating up to 4.4g/kg (2g/lb) of body weight has no bearing on body composition even when consuming 800 more calories per day [10].

Overall, the weight of evidence suggests that 20 g of protein in one sitting is not the upper limit for signaling muscle protein synthesis and therefore, muscle growth. If this is the case, how much should we be eating each meal to maximize the building of muscle?

How Much Protein Per Meal Maximizes Muscle Growth?

How Much Protein To Maximize Muscle

Research suggests that 1.6g/kg (0.75g/lb) of body weight is enough to improve muscle gain and strength in resistance training individuals [11]. However, researchers also suggest that 2.2g/kg (1g/lb) of body weight can be used when wanting to maximize adaptations from resistance training [11].

These numbers are assuming you are eating at maintenance calories. If you are dieting to lose body fat, then higher intakes of 2.3-3.1g/kg (1.05-1.4g/lb) of body weight are recommended to retain muscle mass [9].

If you are eating to gain muscle mass, then you may be able to err on the lower side of the protein intake range.

 It turns out that when in a caloric surplus, energy supplied from carbohydrates and fats is protein sparing [13].

This means you can eat less protein and still maximize increases in lean body mass. That's great news for those struggling to get down pounds of meat each day. And these protein recommendations fit both males and females.

When having your post-workout shake, mixing your protein with a carbohydrate source may be better than just protein as some research has suggested the combination enhances protein synthesis to a greater extent than just protein [12].

Protein Myth Busted

The myth of only being able to absorb 20-30 g of protein in one sitting has been busted. It’s just not true. So next time a bro starts lecturing you on protein intake, show them this article.


1. Moore, D. R., Robinson, M. J., Fry, J. L., Tang, J. E., Glover, E. I., Wilkinson, S. B., ... & Phillips, S. M. (2009). Ingested protein dose response of muscle and albumin protein synthesis after resistance exercise in young men. The American journal of clinical nutrition, 89(1), 161-168.

2. Schoenfeld, B. J., & Aragon, A. A. (2018). How much protein can the body use in a single meal for muscle-building? Implications for daily protein distribution. Journal of the International Society of Sports Nutrition, 15(1), 1-6.

3. Areta, J. L., Burke, L. M., Ross, M. L., Camera, D. M., West, D. W., Broad, E. M., ... & Coffey, V. G. (2013). Timing and distribution of protein ingestion during prolonged recovery from resistance exercise alters myofibrillar protein synthesis. The Journal of physiology, 591(9), 2319-2331.

4. Bandegan, A., Courtney-Martin, G., Rafii, M., Pencharz, P. B., & Lemon, P. W. (2017). Indicator amino acid–derived estimate of dietary protein requirement for male bodybuilders on a nontraining day is several-fold greater than the current recommended dietary allowance. The journal of nutrition, 147(5), 850-857.

5. Macnaughton, L. S., Wardle, S. L., Witard, O. C., McGlory, C., Hamilton, D. L., Jeromson, S., ... & Tipton, K. D. (2016). The response of muscle protein synthesis following whole‐body resistance exercise is greater following 40 g than 20 g of ingested whey protein. Physiological reports, 4(15), e12893.

6. Arnal, M. A., Mosoni, L., Boirie, Y., Houlier, M. L., Morin, L., Verdier, E., ... & Mirand, P. P. (1999). Protein pulse feeding improves protein retention in elderly women. The American journal of clinical nutrition, 69(6), 1202-1208.

7. Soeters, M. R., Lammers, N. M., Dubbelhuis, P. F., Ackermans, M., Jonkers-Schuitema, C. F., Fliers, E., ... & Serlie, M. J. (2009). Intermittent fasting does not affect whole-body glucose, lipid, or protein metabolism. The American journal of clinical nutrition, 90(5), 1244-1251.

8. Moro, T., Tinsley, G., Bianco, A., Marcolin, G., Pacelli, Q. F., Battaglia, G., ... & Paoli, A. (2016). Effects of eight weeks of time-restricted feeding (16/8) on basal metabolism, maximal strength, body composition, inflammation, and cardiovascular risk factors in resistance-trained males. Journal of translational medicine, 14(1), 1-10.

9. Helms, E. R., Zinn, C., Rowlands, D. S., & Brown, S. R. (2014). A systematic review of dietary protein during caloric restriction in resistance trained lean athletes: a case for higher intakes. International journal of sport nutrition and exercise metabolism, 24(2), 127-138.

10. Antonio, J., Peacock, C. A., Ellerbroek, A., Fromhoff, B., & Silver, T. (2014). The effects of consuming a high protein diet (4.4 g/kg/d) on body composition in resistance-trained individuals. Journal of the International Society of Sports Nutrition, 11(1), 1-6.

11. Morton, R. W., Murphy, K. T., McKellar, S. R., Schoenfeld, B. J., Henselmans, M., Helms, E., ... & Phillips, S. M. (2018). A systematic review, meta-analysis and meta-regression of the effect of protein supplementation on resistance training-induced gains in muscle mass and strength in healthy adults. British journal of sports medicine, 52(6), 376-384.

12. Phillips, S. M. (2011). The science of muscle hypertrophy: making dietary protein count. Proceedings of the nutrition society, 70(1), 100-103.

13. Jéquier, E. (1991). Effect of different levels of carbohydrate, fat and protein intake on protein metabolism and thermogenesis. In Protein–Energy Interactions I/D/E/C/G Workshop; October (pp. 21-25).

About the Author

I am a professional strength & conditioning coach that works with professional and international teams and athletes. I am a published scientific researcher and have completed my Masters in Sport & Exercise Science. I've combined my knowledge of research and experience to bring you the most practical bites to be applied to your training.

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